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2500x VFRC vs. HR-dark field (HD 1080/50p) video microscopy of Borrelia sp

Published on Dec 4, 2012

Borrelia spirochete species can hardly be visualized in vital blood due to fast movement and halo effects of conventional dark field illumination. Here we show an improved VFRC (0:00-03:40) and HR (high time and image resolution) dark field (DF) illumination (04:00) using a special condensor with high intensity white LED and a VFRC 110x objective as well as 25-40x zoom lens focussed on a high resolution high sensitivity aps-c cmos chip. Large immune complexes of antibody-precipitated spirochetes, cyst forms of spirochetes, intracellular spirochetes and single vital fast moving spirochetes compromised by antibodies can well be seen in HR-DF, less in VFRC of the freshly isolated capillary blood. The patient is permanently treated by bioresonance, light therapy and herbal remedy therapy specific for neuroborreliosis, according to a novel protocol. Weekly DNA-PCR-tests are performed to screen the blood concentration of borrelia bacteria. The patient is suffering from skin release of spirochete cysts (histiocytomes and leucocytomes) as well as alzheimer-like psycho-neurological disorders. VFRC microscopy and HR-DF are a novel technique invented by Armin Koroknay, Switzerland

Novel spirochetes isolated from mosquitoes and black flies in the Czech Republic.
Sikutová S, Halouzka J, Mendel J, Knoz J, Rudolf I.
J Vector Ecol. 2010 Jun;35(1):50-5.
Institute of Vertebrate Biology, v.v.i., Academy of Sciences of the Czech Republic, Kvetná 8, CZ-603 65 Brno, Czech Republic.
Abstract
During the years 1999-2002, a total of 4,898 individuals of 26 species of hematophagous insects (4,149 mosquitoes, 583 black flies, and 166 tabanid flies) was examined for the presence of spirochetes using dark-field microscopy. There was an overall recovery of spirochetes from the midguts of Culicidae and Simuliidae of 23.5% and 11.4%, respectively. Spirochetes were not detected in Tabanidae. Seven spirochetal strains have been successfully recovered from mosquitoes and black flies: BR149 (Culex pipiens), BR151 (Cx. pipiens), BR173 (Cx. pipiens), BR177 (Cx. pipiens), BR193 (Aedes cinereus), BR208 (Cx. pipiens), and BR231 (Simulium noelleri). The strains have been adapted to laboratory conditions (BSK-H Complete medium). Their preliminary determination based on 16S rRNA gene sequencing has shown that they differ from the Lyme disease spirochete Borrelia burgdorferi sensu lato as well as other members of the Order Spirochaetales indicating novel bacterial species in the Family Spirochaetaceae.

See link here:

http://www.ncbi.nlm.nih.gov/m/pubmed/20618647/

Filament formation associated with spirochetal infection: a comparative approach to Morgellons disease
Perspectives
(3297) Views  (1178) Full article downloads
Authors: Middelveen MJ, Stricker RB
Published Date November 2011
Volume 2011:4 Pages 167 – 177
DOI: http://dx.doi.org/10.2147/CCID.S26183

Marianne J Middelveen, Raphael B Stricker
International Lyme and Associated Diseases Society, Bethesda, MD, USA

Abstract: Bovine digital dermatitis is an emerging infectious disease that causes lameness, decreased milk production, and weight loss in livestock. Proliferative stages of bovine digital dermatitis demonstrate keratin filament formation in skin above the hooves in affected animals. The multifactorial etiology of digital dermatitis is not well understood, but spirochetes and other coinfecting microorganisms have been implicated in the pathogenesis of this veterinary illness. Morgellons disease is an emerging human dermopathy characterized by the presence of filamentous fibers of undetermined composition, both in lesions and subdermally. While the etiology of Morgellons disease is unknown, there is serological and clinical evidence linking this phenomenon to Lyme borreliosis and coinfecting tick-borne agents. Although the microscopy of Morgellons filaments has been described in the medical literature, the structure and pathogenesis of these fibers is poorly understood. In contrast, most microscopy of digital dermatitis has focused on associated pathogens and histology rather than the morphology of late-stage filamentous fibers. Clinical, laboratory, and microscopic characteristics of these two diseases are compared.

Download full Article @ dovepress here:

http://www.dovepress.com/filament-formation-associated-with-spirochetal-infection-a-comparative-peer-reviewed-article-CCID